Stability – Isoelectric Precipitate

The Stability – Isoelectric Precipitate track predicts regional instability by identifying uncharged regions at a given pH, which have an increased potential to aggregate if surface exposed. This method uses the pKa tables from Lehninger et al., 2005.

To apply this track to the sequence:

In the Tracks panel, expand Stability and check the box next to Stability – Isoelectric Precipitate. The track will now be visible in the Sequences view.

To edit track options:

Select the track in the Tracks panel. Open the Track Options section, which appears as follows:

• Residues to average – number of residues to be averaged when constructing each point in the plot. The default is 7.

• pH – MegAlign Pro highlights regions with a pI ± 0.5 pH units from the pH specified here. The default is 7.0.

Click if you wish to return to the default settings.