Part A: Create a templated assembly in SeqMan NGen

To evaluate the effect of a variant on protein structure, the first step is to create a templated assembly in SeqMan NGen.

Create an assembly using the genome template package Homo_sapiens-GRCh38-Ensembl-dbSNP150.zip. This template contains the mapping of the sequences of PDB structures to the human genomic coordinates, and will later allow SeqMan Pro to communicate with Protean 3D. SeqMan NGen outputs an .astar and an .assembly package to use in later steps.

Continue to Part B (optional): Filter for variants of interest in ArrayStar or Part C: View variants in SeqMan Pro.