Introduce a codon change or mutation

To introduce a codon change or mutation:

1. Create or import primers or primer pairs.

2. Open the Primer Design view.

3. Select the Introduce Codon Change/Mutation tool ( ) at the top left of the window; or choose Priming > Introduce Codon Change/Mutation.

4. Click on the codon of interest to open a codon menu showing the current codon checked. The menu lists synonymous codons and other amino acids for introducing non-synonymous changes.

5. Select an alternate codon (for silent mutations), or an alternate amino acid (for non-silent mutations). Selecting an alternate amino acid will replace the sequence via the least disruptive alternate codon. Codons which have been changed cause an edit to the primer, with changes appearing in magenta.