Modify a base in the primer

To modify a base in the primer:

1. Create or import primers or primer pairs.

2. Modify bases using either of these methods:

• • For single primers only (i.e., the primer cannot be part of a primer pair) – From within the Primers view, click inside the Primer Sequence column and edit the existing nucleotide sequence.
    
    
  • For single or paired primers – From within the Primer Design view, click on the Selection Mode tool ( ) at the top left of the window. Overwrite the nucleotide character with the desired character. The edited nucleotide and the resulting changes in translation are shown in magenta. Restriction enzymes whose recognition sites are eliminated by the edit are shown in blue; sites that are introduced by the edit are shown in magenta.