Step 1: Start a cloning project and specify the insert

The first step in performing a cloning simulation is to specify the insert. The following topics describe six different methods of specifying the insert to use with your cloning project:

• Option A – Use a sequence file on your computer as the insert

• Option B – Copy inserts from another SeqBuilder Pro cloning project

• Option C – Use an entire open SeqBuilder Pro sequence as the insert

• Option D – Use part of an open SeqBuilder Pro sequence file as the insert

• Option E – Use the PCR product of the current primer pair as the insert

• Option F – Use restriction enzyme cut sites to create the insert