Modify a base in the primer

To modify a base in the primer:

1. Create or import primers or primer pairs.

2. Modify bases using either of these methods:
   
   
   • For single primers only (i.e., the primer cannot be part of a primer pair) – From within the Primers view, click inside the Primer Sequence column and edit the existing nucleotide sequence.
     
     
   • For single or paired primers – From within the Primer Design view, click on the Selection Mode tool ( ) at the top left of the window. Overwrite the nucleotide character with the desired character.

After you modify a base in the primer, restriction sites are color-coded as follows (see image below):

• Red – Enzyme recognition sites that are are introduced by the edit. In other words, red sites are present in the primer, but not the template.

• Blue – Enzyme recognition sites that are eliminated by the edit. In other words, blue sites are present in the template, but absent in the primer.

• Black – Enzyme recognition sites that are present in both the template and the primer.