Create and manage sets of enzymes

Enzyme selectors provide a way to define a group of restriction enzymes based on specific criteria. The following table shows tasks associated with creating and editing enzyme selectors.

Task	How To
Edit an existing selector	Choose Enzymes > Enzyme Selector Manager.
Create a new selector	Open the manager using Enzymes > New Selector. See the topic Enzyme Selector Manager for information on creating selectors in that dialog.
Create a new selector for enzymes that do/don’t cut a selected region	Select a region of sequence and choose Enzymes > Show Enzymes That Cut the Selection or Enzymes > Show Enzymes That Don’t Cut the Selection. Note that if you apply multiple selectors to the sequence, only the enzymes that meet the criteria for all applied selectors will be displayed. For example, if the Unique Sites selector is already applied to your sequence, and you then select a region and choose Enzymes > Show Enzymes That (Don’t) Cut the Selection, the resulting enzymes will be those that are both unique and cut (or don’t cut) within the selected range.
Create a new selector containing all the enzymes currently checked in the Enzymes panel	Apply a selector using the Enzymes tool () and/or check the desired enzymes in the Enzymes panel, then choose Enzymes > Create a selector from the Checked Enzymes.
Create a new selector for enzymes compatible with a selected enzyme	Double-click on an enzyme to select it. Then choose Enzymes > Show Enzymes Compatible With. Alternatively, right-click on an enzyme in a view and choose Show Compatible Enzymes. The new selector is named after the enzyme you chose.

Once a selector has been created, it will appear in the list of preset enzyme categories that appears when you click the Enzymes tool (). Saved enzyme selectors are available for use in SeqBuilder Pro or GeneQuest.