Option A – Use a sequence file on your computer as the insert

1. From the Cloning menu, select one of the following commands: Gibson, LIC Cloning, In-Fusion Cloning, GeneArt Cloning, Golden Gate, MultiSite Gateway Pro Cloning, Gateway Expression Clone, Gateway Entry Clone, Restriction Enzyme Cloning, TA Cloning, or Directional TOPO Cloning.

2. In the Project tab on the left of the Project window, right-click on Inserts and choose Import.

3. Make a selection in the file chooser and click Open.

4. Expand the Inserts folder, if necessary, by clicking on the plus sign.

5. Drag the inserts from the Inserts folder and drop them in the Fragments section near the bottom right of the Project window.

or…

1. Open your computer’s file explorer and locate the insert sequence(s) of interest.

2. In SeqBuilder Pro, choose File > New Cloning Project.

3. Drag each insert from the file explorer to the Inserts folder of the Project window.

4. Expand the Inserts folder, if necessary, by clicking on the plus sign. Drag the inserts from the Inserts folder and drop them in the Fragments section near the bottom right of the window.

Proceed to Step 2: Specify the method and options.