Add sequences from your computer or the cloud

Some wizard screens require you to add at least one sequence or genome template before proceeding. In these cases, the “Next” button only appears after the item has been added.

• To add one or more sequences from your computer (i.e. “local” sequences), press Add or Add Folder. Navigate to and select the desired file(s) or folder of files, then click Open.

*Note: If your reference sequence is a GFF feature file, with or without a separate FASTA file, we recommend adding the GFF file first. If the FASTA is built into the GFF file, you don’t need to do anything further. If the FASTA file is separate, SeqMan NGen will automatically prompt you to add the accompanying FASTA file.

• To add one or more sequences from the DNASTAR Cloud Data Drive, press Add from Cloud or Add Folder from Cloud. Navigate to and select the desired file(s) or folder of files, then click the green check mark ()

The first time you press a button associated with Cloud Assemblies (or the Cloud Data Drive), you may be prompted to enter your Email and Password. Enter the information that you use when logging in to the DNASTAR website. Check the Save password box if you would like SeqMan NGen to save this information so you do not need to retype it in the future. If you are a licensed Cloud user, a message will display the number of assemblies remaining on your license. Click OK to close the message and continue to the next screen. If you are not licensed, a different message will appear, asking you to purchase a Cloud license through DNASTAR Support (support@dnastar.com).

The following brief video shows how easy it is to upload your data and then perform an assembly on the cloud: