The following table describes each of the workflows available in the Metagenomics tab of the Workflow screen.
| Group | Workflow | Description |
|---|---|---|
| ABI / Sanger | De novo | Cluster metagenomic and other “mixed” sequence samples into contigs. This workflow allows optional removal of specified host DNA. The default parameters for this workflow have been optimized to take into account the short read lengths and presence of repetitive DNA sequences common to metagenomic and 16S rRNA data. |
| Reference-guided | Align/sort metagenomic and other mixed sequence samples to a set of reference sequences, including microbial genome database. This workflow allows optional removal of host DNA before assembling/aligning the remaining reads onto one or more reference sequences/templates. The default parameters for this workflow have been optimized to take into account the short read lengths and presence of repetitive DNA sequences common to metagenomic and 16S rRNA data. | |
| NGS-based | De novo | Cluster metagenomic and other “mixed” sequence samples into contigs. This workflow allows optional removal of host DNA. The default parameters for this workflow have been optimized to take into account the short read lengths and presence of repetitive DNA sequences common to metagenomic and 16S rRNA data. |
| Reference-guided | Align/sort metagenomic and other mixed sequence samples to a set of reference sequences, including microbial genome database. This workflow allows optional removal of host DNA before assembling/aligning the remaining reads onto one or more reference sequences/templates.. The default parameters for this workflow have been optimized to take into account the short read lengths and presence of repetitive DNA sequences common to metagenomic and 16S rRNA data. |
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