Reverse Complement Sequence(s)

The Reverse Complement Sequence(s) template, located in the Templates panel, lets you choose a source file and choose the desired file output and location. Later, when you run the script, SeqNinja will create an output sequence that is the reverse complement of the source file.

When you select this template, you will immediately be prompted to select the source (input) file. Once you have done so, the File Type of Reverse Complement wizard will ask you to choose the desired output file type from a list.

Press Run to fill in the template based on your selections and run the script immediately or Make to fill in the template without running.

Choosing Make lets you review the information and make any necessary changes before pressing Run:

• To change the source sequence, use the Choose Sequence(s) button (see Add and modify a sequence).

• To change the output format, make a selection from the Format drop-down menu.

• To specify the name and location in which to save the output (see Specify output format and location) press the Save Results button and select a location.

*Note: If you need to convert a file from .gbk to .fasta and back to .gbk while preserving features that would normally be lost during conversion to .fasta, prepend this command with SeqNinja’s Set Full File Conversion step.

After setting up the template as desired, click Run to run the script and create an output file. Or choose Cancel to exit from the template without running the script.

Example:

LAMCG.seq begins with the string: GGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAA.

Output file LAMCG_rev.fasta contains the reverse complemented string, which ends with: TTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC.