If you are following the templated transcriptome/RNA-Seq workflow (i.e., if you chose Transcriptome/RNA-Seq Assembly in the Choose Assembly Workflow screen, and Templated assembly in the Choose Assembly Type screen), output results are saved in an .assembly package folder labeled with the user-specified project name and the suffix _RNA-Seq. This folder contains the following files:
Subfolder Name |
File Name |
Description |
|
[project name].astar |
An ArrayStar project file containing data from all of the samples. |
|
[project name].script |
The main script for a templated assembly project. It can be rerun from the SeqMan NGen welcome screen by selecting Re-run Local Assembly. |
|
[project name].table.txt |
The table containing project descriptions and corresponding files; used by the main script. |
|
[project name].template.script |
The templated XNG script, which is applied to all projects listed in [project name].table.txt. |
|
[project name].astar.script |
The script file executed by ArrayStar when the Launch in ArrayStar button is pressed in the Project Report dialog. |
|
query‘n’.script |
The script containing query sequences. This script is generated only if a multiple project assembly includes groups that contain non-equal numbers of files. |
|
_tableScript.template.script |
The script template, executed by the XNG assembler in order to run the QNG assembler. This script is not present before the run and is generated by XNG. |
|
[project name]-qng.script |
After SeqMan NGen finishes running the XNG part of the assembly, QNG assembly is performed using this script. In multi-sample projects, this file references [project name].table.txt. |
|
[project name].Results.txt |
A tab-separated table showing the assembly results. |
Sample‘n’.assembly |
sample‘n’-0.isoforms-features |
Each .assembly project contains a set of files relating to a single contig. |
sample‘n’-0.genes-features | ||
sample‘n’-0.bam | ||
sample‘n’-0.coverage | ||
Sample’n’-Reports |
‘n’-0.report.txt |
A summary of the run; also shown in the Project Report dialog. |