Reference-Based Transcriptome/RNA-Seq Workflow Output

If you are following the templated transcriptome/RNA-Seq workflow (i.e., if you chose Transcriptome/RNA-Seq Assembly in the Choose Assembly Workflow screen, and Templated assembly in the Choose Assembly Type screen), output results are saved in an .assembly package folder labeled with the user-specified project name and the suffix _RNA-Seq. This folder contains the following files:

 

Subfolder Name

File Name

Description

 

[project name].astar

An ArrayStar project file containing data from all of the samples.

 

[project name].script

The main script for a templated assembly project. It can be rerun from the SeqMan NGen welcome screen by selecting Re-run Local Assembly.

 

[project name].table.txt

The table containing project descriptions and corresponding files; used by the main script.

 

[project name].template.script

The templated XNG script, which is applied to all projects listed in [project name].table.txt.

 

[project name].astar.script

The script file executed by ArrayStar when the Launch in ArrayStar button is pressed in the Project Report dialog.

 

query‘n’.script

The script containing query sequences. This script is generated only if a multiple project assembly includes groups that contain non-equal numbers of files.

 

_tableScript.template.script

The script template, executed by the XNG assembler in order to run the QNG assembler. This script is not present before the run and is generated by XNG.

 

[project name]-qng.script

After SeqMan NGen finishes running the XNG part of the assembly, QNG assembly is performed using this script. In multi-sample projects, this file references [project name].table.txt.

 

[project name].Results.txt

A tab-separated table showing the assembly results.

Sample‘n’.assembly

sample‘n’-0.isoforms-features

Each .assembly project contains a set of files relating to a single contig.

sample‘n’-0.genes-features

sample‘n’-0.bam

sample‘n’-0.coverage

Sample’n’-Reports

‘n’-0.report.txt

A summary of the run; also shown in the Project Report dialog.